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ephrinb3 mouse fc chimeric proteins (R&D Systems)

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R&D Systems ephrinb3 mouse fc chimeric proteins
Ephrinb3 Mouse Fc Chimeric Proteins, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ephrinb3+fc/Recombinant+Mouse+Ephrin-B3+Fc+Chimera+Protein%2C+CF/pmc11796360-123-20-26
Average 93 stars, based on 5 article reviews

ephrinb3 mouse fc chimeric proteins - by Bioz Stars, 2026-09

93/100 stars

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Recombinant:

Article Title: Bidirectional EphrinB3/EphA4 Signaling Mediates the Segregation of Medial Ganglionic Eminence- and Preoptic Area-Derived Interneurons in the Deep and Superficial Migratory Stream
Article Snippet: .. Then 5 μg/ml recombinant EphB1-Fc, EphA4-Fc, or ephrinB3-Fc (R&D Systems) clustered with 20 μg/ml Alexa 488-conjugated anti-human IgG antibody (Invitrogen) was applied for 2 h at 37°C and 5% CO 2 in fresh medium. ..

Article Title: Bidirectional EphrinB3/EphA4 Signaling Mediates the Segregation of Medial Ganglionic Eminence- and Preoptic Area-Derived Interneurons in the Deep and Superficial Migratory Stream
Article Snippet: .. Then 5 g/ml recombinant EphB1-Fc, EphA4-Fc, or ephrinB3-Fc (R&D Systems) clustered with 20 g/ml Alexa 488- conjugated anti-human IgG antibody (Invitrogen) was applied for 2 h at 37°C and 5% CO2 in fresh medium. ..

Incubation:

Article Title: Synaptic Function for the Nogo-66 Receptor NgR1: Regulation of Dendritic Spine Morphology and Activity-Dependent Synaptic Strength
Article Snippet: .. For cross-linking experiments, NgR1-Fc, FGF receptor (FGFR)1 (IIIb)/Fc, TROY-Fc, or ephrinB3-Fc (R&D Systems) (each at 13 nM), were incubated with 40 nM 125I-FGF2 (specific activity, 50 Ci/ g; MP Biochemicals, Irvine, CA) with or without the indicated concentrations of cold FGF2 or insulin for 2 h at RT in PBS. .. Crosslinking was initiated by adding BS 3 (bis[sulfosuccinimidyl]suberate) (Pierce, Rockford, IL) to a final concentration of 2 mM and incubating for 30 min at RT.

Activity Assay:

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( A, C, D ) Orthogonal views of SC activation with clustered-EphrinB3 shows that EphrinB3 binds EphB6 ( A ), EphA4 ( C ) and EphB1 ( D ) receptors on SC. ( B ) Western blot confirming the presence of these receptors in SC. ( E,F ) Bound EphrinB3 activation of EphA4 and EphB receptors viewed by immunodetection of phosphorylated forms. ( G,H ) Western blot illustrating that SC incubation with myelin increased the phosphorylation of EphA4 ( G ) and EphB1+B2 ( H ) which was not the case when myelin was previously blocked by anti-EphrinB3 (O.D. p-EphB1+2/GAPDH: one-way ANOVA p=0.003, F(2,15)=8.57, followed by a Tukey’s multiple comparison test); and O.D. of p-EphA4/GAPDH: one-way ANOVA p=0.0036, F(2,15)=8.44, followed by a Tukey’s multiple comparison test). Data are expressed as ratio of the optical density (O.D.) of the bands (mean values ± SD) from 3 independent experiments, control (n=6), myelin (n=6), myelin+anti-EphrinB3 (n=6). Scale bar 20 µm.

Journal: bioRxiv

Article Title: Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling

doi: 10.1101/498261

Figure Lengend Snippet: ( A, C, D ) Orthogonal views of SC activation with clustered-EphrinB3 shows that EphrinB3 binds EphB6 ( A ), EphA4 ( C ) and EphB1 ( D ) receptors on SC. ( B ) Western blot confirming the presence of these receptors in SC. ( E,F ) Bound EphrinB3 activation of EphA4 and EphB receptors viewed by immunodetection of phosphorylated forms. ( G,H ) Western blot illustrating that SC incubation with myelin increased the phosphorylation of EphA4 ( G ) and EphB1+B2 ( H ) which was not the case when myelin was previously blocked by anti-EphrinB3 (O.D. p-EphB1+2/GAPDH: one-way ANOVA p=0.003, F(2,15)=8.57, followed by a Tukey’s multiple comparison test); and O.D. of p-EphA4/GAPDH: one-way ANOVA p=0.0036, F(2,15)=8.44, followed by a Tukey’s multiple comparison test). Data are expressed as ratio of the optical density (O.D.) of the bands (mean values ± SD) from 3 independent experiments, control (n=6), myelin (n=6), myelin+anti-EphrinB3 (n=6). Scale bar 20 µm.

Article Snippet: Mouse EphrinB3-Fc fragments and human Fc were purchased by R&D Systems.

Techniques: Activation Assay, Western Blot, Immunodetection, Incubation, Comparison

( A 1 ) Diagram of the adhesion assay. Coverslips divided by silicon strips were coated with PBS or myelin in each half. After strip removal, SC pre-incubated with Fc or EphrinB3 were seeded homogenously on the coverslip. ( A 2 ) Unclustered EphrinB3 partially reverted myelin-induced inhibition of SC adhesion and spreading, scale bar 200 µm. ( B ) Quantification of the ratio of adhered or round GFP + SC on Myelin over PBS after pre-incubation with unclustered EphrinB3 or Fc. ( C ) SC seeded on substrate coated with increased concentrations of EphrinB3, adhered and spread less in a dose dependent manner respect to the intra-coverslip control substrate (Fc). EphrinB3/Fc: 2.5µg/mL, 10µg/mL, and 20µg/mL. ( D, E ) Quantification of the number of adhered and polarized SC pre-incubated with anti-EphB6 or anti-EphA4 (extracellular domain). Pre-incubation with anti-EpB6 and anti-EphA4 improved the number of adhered and polarized SC on Myelin ( D ) and EphrinB3 ( E ) compared to PBS and Fc respectively. Pre-incubation with IgG as control showed similar results than non-pre-incubated cells ( B, C ). Data are expressed as ratios (mean values ± SD form 3 independent experiments) of Myelin or EphrinB3 surfaces compared to intra-coverslips control non-coated surfaces (PBS) or with equimolar concentration of Fc respectively. In B, C, D, E */** are used for comparison of a group with its hypothetical mean: 1 by one sample two-tailed t-test, and #/## for comparison between two different groups by two-tailed Mann Whitney test (n=7-11 per group) */# means p<0.05; **/## means p<0.01.

Journal: bioRxiv

Article Title: Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling

doi: 10.1101/498261

Figure Lengend Snippet: ( A 1 ) Diagram of the adhesion assay. Coverslips divided by silicon strips were coated with PBS or myelin in each half. After strip removal, SC pre-incubated with Fc or EphrinB3 were seeded homogenously on the coverslip. ( A 2 ) Unclustered EphrinB3 partially reverted myelin-induced inhibition of SC adhesion and spreading, scale bar 200 µm. ( B ) Quantification of the ratio of adhered or round GFP + SC on Myelin over PBS after pre-incubation with unclustered EphrinB3 or Fc. ( C ) SC seeded on substrate coated with increased concentrations of EphrinB3, adhered and spread less in a dose dependent manner respect to the intra-coverslip control substrate (Fc). EphrinB3/Fc: 2.5µg/mL, 10µg/mL, and 20µg/mL. ( D, E ) Quantification of the number of adhered and polarized SC pre-incubated with anti-EphB6 or anti-EphA4 (extracellular domain). Pre-incubation with anti-EpB6 and anti-EphA4 improved the number of adhered and polarized SC on Myelin ( D ) and EphrinB3 ( E ) compared to PBS and Fc respectively. Pre-incubation with IgG as control showed similar results than non-pre-incubated cells ( B, C ). Data are expressed as ratios (mean values ± SD form 3 independent experiments) of Myelin or EphrinB3 surfaces compared to intra-coverslips control non-coated surfaces (PBS) or with equimolar concentration of Fc respectively. In B, C, D, E */** are used for comparison of a group with its hypothetical mean: 1 by one sample two-tailed t-test, and #/## for comparison between two different groups by two-tailed Mann Whitney test (n=7-11 per group) */# means p<0.05; **/## means p<0.01.

Article Snippet: Mouse EphrinB3-Fc fragments and human Fc were purchased by R&D Systems.

Techniques: Cell Adhesion Assay, Stripping Membranes, Incubation, Inhibition, Concentration Assay, Comparison, Two Tailed Test, MANN-WHITNEY

( A ) Quantification of the number of adhered and polarized SC seeded on substrate coated with EphrinB3 with or without FN. Data are expressed as ratio respect to Fc-coated surfaces. EphrinB3 (10µg/mL), EphrinB3 (10µg/mL)+FN (2µg/cm 2 ), n=7 per group. ( B 1 ) Diagram of the adhesion assay on FN. Coverslips divided by silicon strips were coated with Fc+FN (control) or EphrinB3+FN in each half. After strip removal, cells pre-incubated with IgG or anti-Integrinβ1 were seeded homogenously on the coverslip. ( B 2 ) Blocking Integrinβ1 decreased SC adhesion and polarization on EphrinB3+FN compared with Fc+FN, scale, bar 200µm. ( C ) Quantification of SC adhesion and polarization on EphrinB3 when SC are pre-incubated with IgG or anti-integrinß1, n=15-17 per group. ( D-E ) Western blot analysis shows increased expression of Integrinβ1 when SC are activated by myelin extracts. One-way ANOVA p=0.011, F(2,12)=6.62, followed by Tukey’s multiple comparisons test p<0,05, n=5 per group. In A, C */** are used for comparison of a group with its hypothetical mean: 1 by one sample two-tailed t-test, and #/## for comparison between two different groups by two-tailed Mann Whitney test.*/# means p<0.05; **/## means p<0.001.

Journal: bioRxiv

Article Title: Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling

doi: 10.1101/498261

Figure Lengend Snippet: ( A ) Quantification of the number of adhered and polarized SC seeded on substrate coated with EphrinB3 with or without FN. Data are expressed as ratio respect to Fc-coated surfaces. EphrinB3 (10µg/mL), EphrinB3 (10µg/mL)+FN (2µg/cm 2 ), n=7 per group. ( B 1 ) Diagram of the adhesion assay on FN. Coverslips divided by silicon strips were coated with Fc+FN (control) or EphrinB3+FN in each half. After strip removal, cells pre-incubated with IgG or anti-Integrinβ1 were seeded homogenously on the coverslip. ( B 2 ) Blocking Integrinβ1 decreased SC adhesion and polarization on EphrinB3+FN compared with Fc+FN, scale, bar 200µm. ( C ) Quantification of SC adhesion and polarization on EphrinB3 when SC are pre-incubated with IgG or anti-integrinß1, n=15-17 per group. ( D-E ) Western blot analysis shows increased expression of Integrinβ1 when SC are activated by myelin extracts. One-way ANOVA p=0.011, F(2,12)=6.62, followed by Tukey’s multiple comparisons test p<0,05, n=5 per group. In A, C */** are used for comparison of a group with its hypothetical mean: 1 by one sample two-tailed t-test, and #/## for comparison between two different groups by two-tailed Mann Whitney test.*/# means p<0.05; **/## means p<0.001.

Article Snippet: Mouse EphrinB3-Fc fragments and human Fc were purchased by R&D Systems.

Techniques: Cell Adhesion Assay, Stripping Membranes, Incubation, Blocking Assay, Western Blot, Expressing, Comparison, Two Tailed Test, MANN-WHITNEY

( A,B ) Exit of SC entrapped in an agarose drop and seeded on FN+Fc ( A ) and FN+EphrinB3 ( B ), scale bar 200µm. ( C ) More SC exit the agarose drop 5h post-seeding (two-way ANOVA with repeated measures: p=0.03, F(1,4)=10.24). ( D ) SC migrate over longer distances from the drop-edge 4h post-seeding (two-way ANOVA with repeated measures: p=0.02, F(1,4)=12.74) on FN+EphrinB3 coated surfaces (n=3 per group). Graphs represent the values of separate experiments (mean±SD). ( E ) Velocity of single SC was measured in different conditions. Only SC seeded on FN+EphrinB3 increase significantly their migration speed. SC velocity (μm/min) in conditions of Fc+FN coating (n=18), FN+EphrinB3 coating (n=18), pre-incubation with IgG on FN coating (n=12), pre-incubation with anti-EphA4 on FN coating (n=15), pre-incubation with IgG on FN+Fc coating (n=18), pre-incubation with anti-EphA4 on FN+EphrinB3 coating (n=18). This increase is reverted when SC are pre-incubated with anti-EphA4 (One-way ANOVA p=0.002, F(5,93)=4.1; two-tailed Mann Whitney test p=0.002 between control FN+Fc and FN+EphrinB3 group). In E (n) represents tracked single cells of 3 different experiments repeated independently. Data are expressed as mean values ± SD. Single cells were tracked from three different independent experiments, ** means p<0.01

Journal: bioRxiv

Article Title: Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling

doi: 10.1101/498261

Figure Lengend Snippet: ( A,B ) Exit of SC entrapped in an agarose drop and seeded on FN+Fc ( A ) and FN+EphrinB3 ( B ), scale bar 200µm. ( C ) More SC exit the agarose drop 5h post-seeding (two-way ANOVA with repeated measures: p=0.03, F(1,4)=10.24). ( D ) SC migrate over longer distances from the drop-edge 4h post-seeding (two-way ANOVA with repeated measures: p=0.02, F(1,4)=12.74) on FN+EphrinB3 coated surfaces (n=3 per group). Graphs represent the values of separate experiments (mean±SD). ( E ) Velocity of single SC was measured in different conditions. Only SC seeded on FN+EphrinB3 increase significantly their migration speed. SC velocity (μm/min) in conditions of Fc+FN coating (n=18), FN+EphrinB3 coating (n=18), pre-incubation with IgG on FN coating (n=12), pre-incubation with anti-EphA4 on FN coating (n=15), pre-incubation with IgG on FN+Fc coating (n=18), pre-incubation with anti-EphA4 on FN+EphrinB3 coating (n=18). This increase is reverted when SC are pre-incubated with anti-EphA4 (One-way ANOVA p=0.002, F(5,93)=4.1; two-tailed Mann Whitney test p=0.002 between control FN+Fc and FN+EphrinB3 group). In E (n) represents tracked single cells of 3 different experiments repeated independently. Data are expressed as mean values ± SD. Single cells were tracked from three different independent experiments, ** means p<0.01

Article Snippet: Mouse EphrinB3-Fc fragments and human Fc were purchased by R&D Systems.

Techniques: Migration, Incubation, Two Tailed Test, MANN-WHITNEY

( A ) SC encountering CNS white matter ( B ) are activated by the myelin-associated EphrinB3 through EphB6-and EphA4-SC receptors. ( C, D ) The activation by phosphorylation of these receptors impairs SC adhesion to white matter and increases SC expression of Integrinβ1 promoting their adhesion to BV extracellular matrix ( E ). Lesions of white matter undergo the formation and/or remodeling of BV which increase expression of ECM adhesion molecules, such as FN, and further facilitate SC mobilization towards the lesion.

Journal: bioRxiv

Article Title: Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling

doi: 10.1101/498261

Figure Lengend Snippet: ( A ) SC encountering CNS white matter ( B ) are activated by the myelin-associated EphrinB3 through EphB6-and EphA4-SC receptors. ( C, D ) The activation by phosphorylation of these receptors impairs SC adhesion to white matter and increases SC expression of Integrinβ1 promoting their adhesion to BV extracellular matrix ( E ). Lesions of white matter undergo the formation and/or remodeling of BV which increase expression of ECM adhesion molecules, such as FN, and further facilitate SC mobilization towards the lesion.

Article Snippet: Mouse EphrinB3-Fc fragments and human Fc were purchased by R&D Systems.

Techniques: Activation Assay, Expressing

EphrinB3 and CCI injury alter neuroblast migration in the subventricular zone (SVZ). The SVZ was dissected from non-injured (a,b) and CCI injured (c,d) wild type (WT) (a,c) and ephrinB3−/− (b,d) mice, and labeled with anti-DCX (red) and anti-CD31 (green) antibodies. In non-injured WT, neuroblasts can be seen predominantly in thick streams (arrowheads) across the dorsal aspect of the SVZ (a). High magnification insets (a′,a″). CCI injury leads to a mixture of tight (arrowheads) and broader streams (arrows) (c). High magnification insets (c′,c″). In ephrinB3−/− mice, neuroblasts are mainly observed in broad streams (b) and little change after CCI injury (d). High magnification insets (b′,b″,d′,d″).

Journal: Stem cell research

Article Title: EphrinB3 restricts endogenous neural stem cell migration after traumatic brain injury

doi: 10.1016/j.scr.2016.09.029

Figure Lengend Snippet: EphrinB3 and CCI injury alter neuroblast migration in the subventricular zone (SVZ). The SVZ was dissected from non-injured (a,b) and CCI injured (c,d) wild type (WT) (a,c) and ephrinB3−/− (b,d) mice, and labeled with anti-DCX (red) and anti-CD31 (green) antibodies. In non-injured WT, neuroblasts can be seen predominantly in thick streams (arrowheads) across the dorsal aspect of the SVZ (a). High magnification insets (a′,a″). CCI injury leads to a mixture of tight (arrowheads) and broader streams (arrows) (c). High magnification insets (c′,c″). In ephrinB3−/− mice, neuroblasts are mainly observed in broad streams (b) and little change after CCI injury (d). High magnification insets (b′,b″,d′,d″).

Article Snippet: Tubing 1-inch-long joined the cannula to mini-osmotic pumps pre-filled 18 h in advance with clustered ephrinB3-Fc or human Fc control molecules (R&D Systems, MN USA).

Techniques: Migration, Labeling

EphrinB3 increases neuroblast migration outside the SVZ and RMS. Immunolabeled sagittal brain section from a WT mouse showing anti-DCX labeled neuroblasts (red) in RMS, CC and perilesional cortex 3 days after CCI injury, while anti-CD31 (green) labeled vessels were used for tissue referencing (a). Stereological cell counts of the RMS (b), rostral CC (c), caudal CC (d) and peri-lesional cortex (e) show a rostral-to-caudal gradient of neuroblasts from the rostral CC towards the caudal CC and injury site. Increased neuroblasts numbers were observed in the rostral CC (c), caudal CC (d) and perilesional cortex (e) in ephrinB3−/− as compared to WT mice. High-magnification images of neuroblasts in the rostral CC (f), caudal CC (g) and perilesional cortex (h). CC, corpus callosum; DG, dentate gyrus; Hipp, Hippocampus; LV, lateral ventricle; OB, olfactory bulb; RMS, rostral migratory stream; SVZ, subventricular zone. *p < 0.05, **p < 0.01 as compared with their respective non-injured controls.

Journal: Stem cell research

Article Title: EphrinB3 restricts endogenous neural stem cell migration after traumatic brain injury

doi: 10.1016/j.scr.2016.09.029

Figure Lengend Snippet: EphrinB3 increases neuroblast migration outside the SVZ and RMS. Immunolabeled sagittal brain section from a WT mouse showing anti-DCX labeled neuroblasts (red) in RMS, CC and perilesional cortex 3 days after CCI injury, while anti-CD31 (green) labeled vessels were used for tissue referencing (a). Stereological cell counts of the RMS (b), rostral CC (c), caudal CC (d) and peri-lesional cortex (e) show a rostral-to-caudal gradient of neuroblasts from the rostral CC towards the caudal CC and injury site. Increased neuroblasts numbers were observed in the rostral CC (c), caudal CC (d) and perilesional cortex (e) in ephrinB3−/− as compared to WT mice. High-magnification images of neuroblasts in the rostral CC (f), caudal CC (g) and perilesional cortex (h). CC, corpus callosum; DG, dentate gyrus; Hipp, Hippocampus; LV, lateral ventricle; OB, olfactory bulb; RMS, rostral migratory stream; SVZ, subventricular zone. *p < 0.05, **p < 0.01 as compared with their respective non-injured controls.

Article Snippet: Tubing 1-inch-long joined the cannula to mini-osmotic pumps pre-filled 18 h in advance with clustered ephrinB3-Fc or human Fc control molecules (R&D Systems, MN USA).

Techniques: Migration, Immunolabeling, Labeling

EphrinB3-Fc infusion reduces neuroblast migration into the overlying CC at 2 days following cannula injury. Immunolabeled sagittal brain section from a WT mouse shows anti-DCX labeled neuroblasts (red) in the RMS, CC and subcortical tissues at 2 days post-injury, while anti-CD31 (green) labeled vessels were used for tissue referencing (a). High-magnification inset of neuroblasts migrating from RMS to CC and subcortical tissues (arrowheads depict neuroblast chains) (a′). Stereology shows increased numbers of neuroblasts in the RMS (b) and CC (c) in Fc-control WT mice as compared with non-injured WT mice. Infusion of ephrinB3-Fc reduced neuroblast numbers in the CC of WT mice (c). EphrinB3−/− mice show increased chain (d) and isolated cell (e) migration in the rostral CC as compared with WT mice. Infusion of clustered ephrinB3-Fc reduced the number of neuroblast chains/clusters but increased the number of isolated neuroblasts. CC, corpus callosum; RMS, rostral migratory stream; SVZ, subventricular zone. *p < 0.05, **p < 0.01, ***p < 0.001 as compared with their respective non-injured controls; #p < 0.05 as compared with their respective Fc controls.

Journal: Stem cell research

Article Title: EphrinB3 restricts endogenous neural stem cell migration after traumatic brain injury

doi: 10.1016/j.scr.2016.09.029

Figure Lengend Snippet: EphrinB3-Fc infusion reduces neuroblast migration into the overlying CC at 2 days following cannula injury. Immunolabeled sagittal brain section from a WT mouse shows anti-DCX labeled neuroblasts (red) in the RMS, CC and subcortical tissues at 2 days post-injury, while anti-CD31 (green) labeled vessels were used for tissue referencing (a). High-magnification inset of neuroblasts migrating from RMS to CC and subcortical tissues (arrowheads depict neuroblast chains) (a′). Stereology shows increased numbers of neuroblasts in the RMS (b) and CC (c) in Fc-control WT mice as compared with non-injured WT mice. Infusion of ephrinB3-Fc reduced neuroblast numbers in the CC of WT mice (c). EphrinB3−/− mice show increased chain (d) and isolated cell (e) migration in the rostral CC as compared with WT mice. Infusion of clustered ephrinB3-Fc reduced the number of neuroblast chains/clusters but increased the number of isolated neuroblasts. CC, corpus callosum; RMS, rostral migratory stream; SVZ, subventricular zone. *p < 0.05, **p < 0.01, ***p < 0.001 as compared with their respective non-injured controls; #p < 0.05 as compared with their respective Fc controls.

Article Snippet: Tubing 1-inch-long joined the cannula to mini-osmotic pumps pre-filled 18 h in advance with clustered ephrinB3-Fc or human Fc control molecules (R&D Systems, MN USA).

Techniques: Migration, Immunolabeling, Labeling, Isolation

Application of ephrinB3 regulates neuroblast chain migration from cultured SVZ explants. Brightfield (a,c,e) and DCX-immunolabeled (b,d,f) SVZ explants show migrating neuroblast in basal (a,b) conditions, unclustered (Uncl.) ephrinB3-Fc (c,d) to block Eph signaling, and clustered (Cl.) ephrinB3-Fc (e,f) to activate Eph signaling. The extent of chain migration and single cell migration were measured using a semi-quantitative scale: 0 = no outgrowth; 1 ≤ 10 chains/cells; 2 = 10–50 chains/cells; 3 = 50–100 chains/cells; 4 = extensive growth. The area of explant outgrowth was measured and expressed as a ratio of outgrowth area (i.e. explant size). Graphs show increased numbers of chains migrating out of the explants after treatment with Uncl. ephrinB3-Fc (g), while isolated cells were increased after treating with Cl. ephrinB3-Fc (h). Both Cl. and Uncl. ephrinB3-Fc increased the total outgrowth of the explants (i). Results in all graphs show mean ± SEM of 39–48 explants per treatment; *p < 0.05 compared to basal conditions.

Journal: Stem cell research

Article Title: EphrinB3 restricts endogenous neural stem cell migration after traumatic brain injury

doi: 10.1016/j.scr.2016.09.029

Figure Lengend Snippet: Application of ephrinB3 regulates neuroblast chain migration from cultured SVZ explants. Brightfield (a,c,e) and DCX-immunolabeled (b,d,f) SVZ explants show migrating neuroblast in basal (a,b) conditions, unclustered (Uncl.) ephrinB3-Fc (c,d) to block Eph signaling, and clustered (Cl.) ephrinB3-Fc (e,f) to activate Eph signaling. The extent of chain migration and single cell migration were measured using a semi-quantitative scale: 0 = no outgrowth; 1 ≤ 10 chains/cells; 2 = 10–50 chains/cells; 3 = 50–100 chains/cells; 4 = extensive growth. The area of explant outgrowth was measured and expressed as a ratio of outgrowth area (i.e. explant size). Graphs show increased numbers of chains migrating out of the explants after treatment with Uncl. ephrinB3-Fc (g), while isolated cells were increased after treating with Cl. ephrinB3-Fc (h). Both Cl. and Uncl. ephrinB3-Fc increased the total outgrowth of the explants (i). Results in all graphs show mean ± SEM of 39–48 explants per treatment; *p < 0.05 compared to basal conditions.

Article Snippet: Tubing 1-inch-long joined the cannula to mini-osmotic pumps pre-filled 18 h in advance with clustered ephrinB3-Fc or human Fc control molecules (R&D Systems, MN USA).

Techniques: Migration, Cell Culture, Immunolabeling, Blocking Assay, Isolation

EphrinB3 is expressed in the human control and TBI brains. Expression of ephrinB3 in uninjured (n = 2) or traumatized (n = 7) human brain samples as detected by Western blotting. The traumatized samples were grouped into tissue collection between 3 and 24 h (acute; n= 6) or >5 days (122 h; n = 1). No differences were observed between acute TBI samples and uninjured controls; however, in the one sample collected from a patient 122 h after injury a reduced level of ephrinB3 was observed despite a high level of expression of β-actin in this sample.

Journal: Stem cell research

Article Title: EphrinB3 restricts endogenous neural stem cell migration after traumatic brain injury

doi: 10.1016/j.scr.2016.09.029

Figure Lengend Snippet: EphrinB3 is expressed in the human control and TBI brains. Expression of ephrinB3 in uninjured (n = 2) or traumatized (n = 7) human brain samples as detected by Western blotting. The traumatized samples were grouped into tissue collection between 3 and 24 h (acute; n= 6) or >5 days (122 h; n = 1). No differences were observed between acute TBI samples and uninjured controls; however, in the one sample collected from a patient 122 h after injury a reduced level of ephrinB3 was observed despite a high level of expression of β-actin in this sample.

Article Snippet: Tubing 1-inch-long joined the cannula to mini-osmotic pumps pre-filled 18 h in advance with clustered ephrinB3-Fc or human Fc control molecules (R&D Systems, MN USA).

Techniques: Expressing, Western Blot

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ephrinb3 mouse fc chimeric proteins (R&D Systems)

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